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Disease. Gummy stem blight. Causes leaf spot, stem canker, black leaf and fruit rot; common name refers to the characteristic gummy exudate. Didymella bryoniae (Auersw.) Rehm [anamorph Phoma cucurbitacearum (Fr.) Sacc.] is the causative agent of gummy stem blight (GSB), a disease affecting. Gummy stem blight of cucurbits produces a variety of symptoms which are referred to as leaf spot, stem canker, vine wilt and black fruit rot. Lesions on leaves.

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Identification and characterization of the causal agent of gummy stem blight from muskmelon and watermelon in East China. Relationship of developmental stage of cantaloupe fruit to black rot susceptibility and enzyme production by Didymella bryoniae.

Crop refuse should be ploughed deeply immediately after harvest to reduce fungus survival. Gummy stem blight, caused by Didymella bryoniae, is one of the most important diseases of cucurbits in many countries of the world Lee et al. The standard management practice for D.

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All other reagents were analytical grade and didymell purchased from Sinopharm Chemical Reagent Co. LAMP can rapidly amplify nucleic acids under isothermal conditions with a set of four to six specially designed primers and the large fragment of Bst DNA polymerase, which undergoes strand displacement activity to amplify target DNA in less than 60 min Notomi et al.

In recent years, the LAMP-based didymeella has grown in popularity for the detection of many plant-pathogenic fungi Niessen and Vogel, ; Denschlag et al. Go to distribution map Suspensions of only ascospores are difficult to prepare but presumably would also be effective. Note that production of pseudothecia is variable among isolates and between laboratories.


A Detecting LAMP products by adding fluorescence metal indicator calcein ; the assessment was based on visualization of a color change from brown to yellowish-green. Currently cultural practices and fungicides hryoniae well in greenhouses and in the field only if D. Diagnostic signs are the pycnidia and pseudothecia of the pathogen that form readily in leaf spots and in lesions on other above-ground plant parts, including petioles, vines, stems, tendrils, and pedicels of flowers and fruit Figs.

Gummy stem blight can occur on seedlings of watermelon, muskmelon, bryooniae other cucurbits grown in greenhouses for use as transplants 11, Author information Article notes Didymellq and License information Disclaimer. Retrieved from ” https: Wild hop also known as white bryony Bryonia alba is susceptible to gummy stem blight; B.

Eventually all infected vines will become necrotic and occasionally the plant dies due to wilting and defoliation [1,7]. Actively expanding leaf spots of gummy stem blight on a bottle gourd Lagenaria siceraria leaf with water-soaked margins due to cell wall-degrading enzymes produced by Didymella bryoniae.

Two pieces cut from didmella established culture are placed around the edge of the filter paper on the agar, not on the filter paper.

Didymella bryoniae

The first symptoms appear as grayish green, circular spots between the veins of the leaf lobes [1]. Rapid, specific, simple, in-field detection of Xanthomonas campestris pathovar musacearum by loop-mediated isothermal amplification.

Plant Materials Muskmelon Cucumis melo L. A positive reaction is indicated by a color change from brown to a dicymella color.


Pseudothecia are dark brown to black and usually are slightly immersed in the host tissue Fig. Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of Sporisorium scitamineum in sugarcane. Cultures on filter paper will remain viable up to 10 years.

Differential susceptibility of nine cucurbit species to the foliar blight and crown canker phases of gummy stem blight.

No use, distribution or reproduction is permitted which does not comply with these terms. Moreover, it can be monitored with the naked eye by adding colorimetric indicators, such as calcein, which produces a green fluorescent signal if the LAMP reaction is positive Zhang et al. This pathogen is also common in greenhouses where cucumbers are grown [2]. Fruiting bodies form readily when diseased tissue is placed in a simple humidity chamber made from a moist paper towel and a self-sealing polyethylene bag and held overnight at ambient temperature and lighting.

AFLP analysis of a worldwide collection of Didymella bryoniae. On jack-o-lantern pumpkin, black rot vryoniae as numerous small or several large, sunken lesions 3.

Didymella bryoniae Fuckel Rehm, LAMP-based assays are growing in popularity, and have been bryonkae to the detection of many plant-pathogenic fungi Niessen, Muskmelon genomic DNA was extracted from the leaves. Role of insect injury and powdery mildew in the epidemiology of the gummy stem blight disease of cucurbits.